Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos

Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos

Knock-in mice with precise insertions are efficiently generated through delivery of CRISPR–Cas9 to two-cell embryos. Rapid, efficient generation of knock-in mice with targeted large insertions remains a major hurdle in mouse genetics. Here, we describe two-cell homologous recombination (2C-HR)-CRISPR, a highly efficient gene-editing method based on introducing CRISPR reagents into embryos at the two-cell stage, which takes advantage of the open …

Role of LADs and Epigenetic Pathways in Nuclear Architecture During Early Mouse Embryo Development

A new study in Cell reveals that lamina-associated domains (LADs) in early mouse embryos are shaped by dynamic epigenetic pathways, highlighting how histone modifications and chromatin structure guide nuclear architecture during development.