Integrated Epigenetic and Genetic Programming of Primary Human T Cells

Epigenetic Reprogramming Platform Offers Path to More Effective T Cell Therapies

Scientists at Arc Institute, Gladstone Institutes, and the University of California, San Francisco (UCSF) have developed an epigenetic editing platform that allows for the safe and simultaneous modification of multiple genes in primary human T cells. Their work, published in Nature Biotechnology, leveraged two RNA-based editing tools called CRISPRoff and CRISPRon that silence or activate genes via epigenetic modifications. “The T cells essential…

Integrated Epigenetic and Genetic Programming of Primary Human T Cells

Investigators developed an all-RNA platform for efficient, durable and multiplexed epigenetic programming in primary human T cells, stably turning endogenous genes off or on using CRISPRoff and CRISPRon epigenetic editors. [Nature Biotechnology] Full Article