From in vitro to in vivo: The Dominant role of PEG-Lipids in LNP performance - Pharma Excipients

From in vitro to in vivo: The Dominant role of PEG-Lipids in LNP performance - Pharma Excipients

Abstract Lipid nanoparticles (LNPs) are widely employed for delivering nucleic acids, such as mRNA, in both vaccines and therapeutic applications. These LNPs generally include an ionisable lipid (typically ∼ 50 %) to facilitate nucleic acid encapsulation and a PEG-lipid (typically ∼ 1.5 %) to enhance nanoparticle stability. To examine how the choice of PEG-lipid impacts LNP performance, we investigated the physicochemical characteristics and pot…