Increasing the Efficiency of Homology-Directed Repair for CRISPR-Cas9-Induced Precise Gene Editing in Mammalian Cells

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells

The efficiency of precise CRISPR/Cas9 genome editing is increased by inhibition of the nonhomologous end joining pathway. The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared with the higher efficiency of the nonhomologous end-joining (NHEJ) pathway. To enhance HDR, enabling the insertion of precise genetic modifications, …

Gene-Editing Breakthrough Saves an Infant with a Rare Disease

CRISPR therapies are moving from proof-of-concept to real-world treatments for a growing list of diseases.

What if we could "rewrite" a plant's DNA to make it more drought-resistant or richer in antioxidants? What if we could correct a faulty gene before a disease occurs...

Small molecule makes RDEB gene editing more accurate: Study

Researchers used a small molecule called M3814 for more accurate editing of genetic mutations causing recessive dystrophic epidermolysis bullosa (RDEB), according to a new study. This method enabled skin cells from three patients to restore their production of type VII collagen (C7) protein, which they lacked, the researchers noted. M3814 works by inhibiting DNA-dependent protein kinase, an enzyme that repairs breaks in DNA. Using M3814, the res…

A baby with a rare and incurable disease became the world's first patient to have its edited DNA, which allowed its recovery to date.