Identification and characterization of a novel β-galactosidase active at low temperatures from the Antarctic fungus Tetracladium sp., expressed in Saccharomyces cerevisiae

Identification and characterization of a novel β-galactosidase active at low temperatures from the Antarctic fungus Tetracladium sp., expressed in Saccharomyces cerevisiae

Backgroundβ-Galactosidases are widely used in the dairy industry to produce lactose-free milk and prebiotics such as galacto-oligosaccharides and lactulose. Since commercial β-galactosidases have optimal activity at 35 to 70 ºC, β-galactosidases that are highly active at low...