Expanding the Genetic Toolkit: Adenine and Cytosine Base Editors for Efficient Gene Disruption in Aspergillus Niger

Expanding the Genetic Toolkit: Adenine and Cytosine Base Editors for Efficient Gene Disruption in Aspergillus Niger

Despite revolutionizing fungal genetic engineering, conventional CRISPR/Cas9-mediated knockouts rely on DNA double-strand breaks (DSBs), which can cause unwanted insertions and deletions, chromosomal abnormalities, and cytotoxicity. Base editors such as adenine base editors (ABEs), which conve...