CRISPR Screen Identifies Gene Editing Barriers

CRISPR Screen Identifies Gene Editing Barriers

A genome-wide screening platform has highlighted cellular barriers that limit the effectiveness of nonviral genome editing, which could be targeted to enhance its potency. The findings, in Nature Communications, suggest that temporarily lowering these barriers could present a window of opportunity through which high-efficiency gene editing could be performed before normal physiology is restored. “These findings shift the paradigm of delivery opt…

Researchers identify genes that hinder CRISPR editing efficiency

Like a delivery driver navigating crowded city streets, a gene-therapy-toting lipid nanoparticle faces a gauntlet of potential detours on its journey toward a cell's nucleus.