Transgene- and Tissue Culture-Free Heritable Genome Editing Using RNA Virus-Based Delivery in Wheat
5 Articles
5 Articles
Transgene- and tissue culture-free heritable genome editing using RNA virus-based delivery in wheat
CRISPR–Cas genome editing technology is a cutting-edge strategy for crop breeding. However, the delivery of genome-editing reagents remains to be a technological bottleneck in monocot plants1. Here we engineered barley yellow striate mosaic virus (BYSMV) into a negative-strand RNA virus-based vector system2 for delivery of both Cas9 and single guide RNA to achieve heritable gene editing in different wheat cultivars. We found that fusion of a mob…
A team of Argentine scientists recently made a discovery that could contribute to increasing wheat yield through genetic improvement strategies. The study led by Fernanda González, a CONICET researcher at the Center for Research and Transfer of the Northwest of the Province of Buenos Aires” (CIT NOBA, CONICET), published in the specialized magazine Field Crops Research, confirmed, through field trials, the identification of two regions of the wh…


Heritable Wheat Genome Editing via RNA Virus Delivery
In a groundbreaking advancement poised to revolutionize the future of crop genetic engineering, scientists have unveiled a novel method for delivering CRISPR–Cas9 genome-editing tools into wheat plants using an RNA virus-based vector. This technological breakthrough addresses a major bottleneck in plant biotechnology — the efficient and heritable delivery of gene-editing components into monocot species, which include important cereal crops such …
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