Comprehensive comparative analysis of 5′-end RNA-sequencing methods

Comprehensive comparative analysis of 5′-end RNA-sequencing methods

Specialized RNA-seq methods are required to identify the 5′ ends of transcripts, which are critical for studies of gene regulation, but these methods have not been systematically benchmarked. We directly compared six such methods, including the performance of five methods on a single human cellular RNA sample and a new spike-in RNA assay that helps circumvent challenges resulting from uncertainties in annotation and RNA processing. We found that…

First clinical RNA sequencing test promises enhanced genetic diagnostics

RNA sequencing has emerged as a powerful supplement to DNA sequencing for Mendelian disease diagnosis, but clinical translation of diagnostic RNA-seq has not been widely achieved. Researchers at Baylor College of Medicine's Medical Genetics and Multiomics Laboratory have published the clinical validation of the first RNA sequencing test for diagnostic whole-transcriptome analysis for genetic disorders.

Evaluation of commercially available small RNASeq library preparation kits using low input RNA - BMC Genomics

Evolving interest in comprehensively profiling the full range of small RNAs present in small tissue biopsies and in circulating biofluids, and how the profile differs with disease, has launched small RNA sequencing (RNASeq) into more frequent use. However, known biases associated with small RNASeq, compounded by low RNA inputs, have been both a significant concern and a hurdle to widespread adoption. As RNASeq is becoming a viable choice for the…