CRISPR/Cas9 creates transgene-free MtABCG46 mutants in Medicago truncatula

CRISPR/Cas9 creates transgene-free MtABCG46 mutants in Medicago truncatula

In a development that could reshape how scientists probe the molecular machinery of legumes, researchers in Poland have created the first transgene-free knockout lines of a key transporter gene in the model legume Medicago truncatula, using a two-stage CRISPR/Cas9 strategy that promises to dramatically accelerate functional genomics in one of agriculture’s most important plant families. […]