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Process Intensification Of Cultivated Meat Production Through Microcarrier Addition Strategy Optimisation
Cell isolation and purification bSCs were isolated as previously described51,52. Briefly, skeletal muscle obtained from slaughtered cattle (male and female, aged from 1 to 7 years) was chopped prior digestion with collagenase (1 h at 37 °C) (CLSAFA, Worthington). Larger pieces of tissues were filtered out using 100 μm nylon mesh cell strainer. Red blood cells contained in the filtrate were lysed with ammonium–chloride–potassium lysis buffer (A94…
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Process Intensification Of Cultivated Meat Production Through Microcarrier Addition Strategy Optimisation
Cell isolation and purification bSCs were isolated as previously described51,52. Briefly, skeletal muscle obtained from slaughtered cattle (male and female, aged from 1 to 7 years) was chopped prior digestion with collagenase (1 h at 37 °C) (CLSAFA, Worthington). Larger pieces of tissues were filtered out using 100 μm nylon mesh cell strainer. Red blood cells contained in the filtrate were lysed with ammonium–chloride–potassium lysis buffer (A94…
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